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产品编号 | 产品名称 | 产品包装 | 产品价格 |
C7204 | NIH/3T3 (小鼠胚胎成纤维细胞) | 1支/瓶 | 1060.00元 |
Organism | Tissue | Morphology | Culture Properties |
Mus musculus (Mouse) | Embryo | Fibroblast | Adherent |
本细胞株详细信息如下:
General Information | |
Cell Line Name | NIH/3T3 (Mouse Embryonic Fibroblast Cells) |
Synonyms | NIH/3T3 (小鼠胚胎成纤维细胞) |
Organism | Mus musculus (Mouse) |
Tissue | Embryo |
Cell Type | Fibroblast |
Morphology | Fibroblast |
Disease | - |
Strain | NIH/Swiss |
Biosafety Level* | 1 |
Age at Sampling | Embryo |
Gender | Male |
Genetics | Whole embryo |
Ethnicity | - |
Applications | This cell line is a suitable transfection host. The NIH/3T3 cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies. |
Category | Spontaneously immortalized cell line |
* Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Characteristics | |
Karyotype | - |
Virus Susceptibility | Murine leukemia virus |
Derivation | - |
Clinical Data | - |
Antigen Expression | - |
Receptor Expression | - |
Oncogene | - |
Genes Expressed | - |
Gene expression databases | GEO: GSM1014177 |
Metastasis | - |
Tumorigenic | - |
Effects | - |
Comments | Tested and found negative for ectromelia virus (mousepox). |
Culture Method | |
Doubling Time | ~20 hrs |
Methods for Passages | Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) |
Medium | DMEM (high glucose)+10% FBS |
Special Remarks | - |
Medium Renewal | Twice per week.DO NOT ALLOW THE CELLS TO BECOME CONFLUENT. |
Subcultivation Ratio | 3~5×10^3 cells/cm2 |
Growth Condition | 95% air+ 5% CO2, 37℃ |
Freeze medium | DMEM (high glucose)+20% FBS+10% DMSO,也可以订购碧云天的细胞冻存液(C0210)。 |
本细胞株经过支原体检测(Mycoplasma Test),检测结果为阴性。
本细胞株经过物种鉴定,鉴定结果为小鼠。
包装清单:
产品编号 | 产品名称 | 包装 |
C7204 | NIH/3T3 (小鼠胚胎成纤维细胞) | 1支/瓶 |
— | 说明书 | 1份 |
保存条件:
对于细胞培养瓶或离心管运输的活细胞,室温3-5天有效。对于干冰运输的冻存细胞,液氮保存,长期有效;-80℃保存,2个月有效。
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