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ompA/ompA蛋白Recombinant Escheri

chia coli Outer membrane protein A (ompA)重组蛋白Outer membrane protein II* (con) (tolG) (tut)蛋白
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  • ¥9720 - 16200
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  • CN
  • CSB-CF364256ENV(A4)
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Escherichia coli Outer membrane protein A (ompA)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      100μg/20μg

    规格:100μg产品价格:¥16200.0
    规格:20μg产品价格:¥9720.0

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    基因名:

    ompA

    Uniprot No.:

    P0A910

    别名:

    Outer membrane protein II* (con) (tolG) (tut)

    种属:

    Escherichia coli (strain K12)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    in vitro E.coli expression system

    分子量:

    38.0 kDa

    表达区域:

    22-346aa

    氨基酸序列:

    APKDNTWYTGAKLGWSQYHDTGFINNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDVVTQPQA

    蛋白标签:

    N-terminal 10xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    CF Transmembrane Protein & In Stock Protein

    Biological_Activity:

    /

    Research Areas:

    Metabolism

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006)

    Function:

    Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes.

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    图标文献和实验
    该产品被引用文献
    "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006)
    相关实验
    • 蛋白纯化攻略分享

      柱体积 30%。 分子筛 膜蛋白的纯化流程中,分子筛是理想化的最终步骤。不仅可以去除聚集体以及与目的蛋白大小不同的杂质,还可以进行缓冲液更换。这些因素往往能够决定后续的结构测定是否顺利。 推荐使用高分辨率分子筛大体积预装柱 Superdex 200 Increase 10/300 GL 和 Superose 6 Increase 10/300 GL。其最大上样量可达 500μL。   案例分析 外膜蛋白 A (Outer membrane protein A, OmpA) 是革兰氏阴性菌外膜蛋白质的主要

    • Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems

      and FimD usher proteins from the bacterial outer membrane, and the purification of PapD-PapG and FimC-FimH chaperone-�subunit complexes from the periplasm. These Pap and Fim proteins belong to the P and type 1 pilus systems of uropathogenic Escherichia coli

    • 最难搞的膜蛋白纯化攻略来了!

      500µl。 案例分析 外膜蛋白 A(Outer membrane protein A, OmpA)是革兰氏阴性菌外膜蛋白质的主要组分,也是一个关键的毒性因子,且与细菌生物膜合成、真核细胞感染、抗生素抗性和免疫调节息息相关。因此可作为一个潜在的药物靶点。 以下是从大肠杆菌中纯化非标签膜蛋白 OmpA 亚单位的流程。 首先使用合适的去垢剂 C8E4,把 OmpA蛋白溶解出来; 然后使用阴离子交换层析柱 RESOURCE Q 1 ml 进行初步纯化,收集目标蛋白

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    ompA/ompA蛋白Recombinant Escherichia coli Outer membrane protein A (ompA)重组蛋白Outer membrane protein II* (con) (tolG) (tut)蛋白
    ¥9720 - 16200