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- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
MicrobiologyUniprot ID
P0A912Gene Names
palAlternative Name(s)
Tol-Pal system lipoprotein Pal (excC) (PAL)Lead Time
3-7 business daysOrganism
Escherichia coli (strain K12)Source
E.coliExpression Region
22-173aaProtein Length
Full Length of Mature ProteinTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
CSSNKNASNDGSEGMLGAGTGMDANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYKVTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDEAAYSKNRRAVLVYMW
24.1 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验RIP技术(RNA结合蛋白免疫沉淀),研究肿瘤发生和转移中microRNA失调的有力工具
, RISC),然后通过不完全的碱基互补靶向到目标mRNA的3’UTR区,再通过引导酶切影响了mRNA的稳定性,或直接阻碍翻译过程,从而介导特异mRNA靶标的转录后基因沉默。 miRNA参与转录后调控网络。miRNA靶向到目标mRNA的3’UTR区,抑制靶mRNA的蛋白表达。(摘自Chatterjee & Pal, Biol Cell. 2009) miRNA对许多生理过程产生重要影响,如细胞生长、凋亡、应激应答、干细胞分化潜能的维持
起来的。Joullie在合成天然环肽生物碱Sanjoinine G1和其C11位对映异构体时在环合步骤中就应用了五氟苯酚酯法。首先以D-丝氨酸为原料,经多步反应得到环合前体,用五氟苯酚活化羧基,N端苄氧羰基经氢解脱掉后,以4-吡咯烷吡啶为催化剂,在二氧六环中回流,最终得到两个互为异构体的混合物,收率分别为27%和22%。另外,海洋生物环肽Patellamine B以及对纤维蛋白酶和丝氨酸蛋白酶有强烈抑制作用的环肽Cyclotheonamide A的环合步骤也是应用了五氟苯酯法,收率分别是20
2、Aub、Piwi 和Ago3; Ago1 与miRNA 偶联,而Ago2 与siRNA偶联,Ago1 和Ago2 组成Ago 亚家族 [29,30]; Aub、Piwi 和Ago3 属于Ago 家族的PIWI 亚家族,与piRNA 偶联。 RISC 还含有其他一些蛋白质组分,包括Vasa 内含子基因蛋白质(VIG)、脆弱X 蛋白的果蝇同源物(DmFXR)、Tudor-SN、潜在的RNA 解旋酶 Dmp68 及Gemin3 等[31]。这些蛋白质成分不是RISC 核酸酶活性必需的,可能
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