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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Chorismate pyruvate-lyase (ubiC)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Chorismate pyruvate-lyase (ubiC)品名简称:
Recombinant E.coli ubiC protein货号:
CSB-EP338764ENV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ubiCUniprot No.:
P26602别名:
CL (CPL)种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
19.5 kDa表达区域:
2-165aa氨基酸序列:
SHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGKTVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSGPELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLPASPLY蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate for the ubiquinone pathway.功能2:
/文献:
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006)研究领域:
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许多靶标的超敏感检测。一、新的BL/CL生物技术工具新的BL/CL报告基因报告基因技术代表了分子生物学最近主要的进展之一。报告基因是一段DNA序列,编码一个很容易被侦测到的蛋白或酶。它们可以人工引入到一个细胞内来监测基因的表达,以得到整体细胞生物传感器或细胞定位的作用。报告基因技术应用到研发BL/CL全细胞生物传感器的原理如图1a。目前,细菌荧光素酶基因(如陆上的Photorhabdus luminescens和海洋中Vibrio harveyi细菌的lux基因),真核的来自于萤火虫Photinus
表达容易形成不溶的聚集体,称为“包涵体”。大肠杆菌表达产生包涵体有以下优点[1,2]: 1) 以包涵体形式表达的蛋白质的浓度比较高,有时甚至可以达到细胞总蛋白含量的30% [3]; 2) 如果重组蛋白质以包涵体形式存在,由于包埋了酶攻击的位点,可以最大限度地抵抗蛋白质酶的攻击; 3) 包涵体表达的蛋白质没有活性,细胞破碎和以后的纯化步骤,不用考虑蛋白质的失活问题; 4) 包涵体形式表达的蛋白质与宿主细胞的其他蛋白质的分离比可溶蛋白质的分离方法简便,造价低,使用简单的离心或者过滤
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