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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Hypoderma lineatum Hypodermin-A
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Hypoderma lineatum Hypodermin-A品名简称:
Recombinant Hypoderma lineatum Hypodermin-A protein货号:
CSB-EP327428HRQ(M)规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P35587别名:
HA种属:
Hypoderma lineatum(Early cattle grub)(Common cattle grub)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
28.8 kDa表达区域:
31-256aa氨基酸序列:
IVGGVESKIEDFPWQISLQRDGRHYCGGSIYSKNVIITAAHCLRNVVAEELRVRVGSSYWEHGGSLRNISKFQIHESYVEPTKEYDVALLKLDSDLSFNSTIKAIELTNEIPPEYADAIVSGWGETLVPPPGIPDQLRSVDVKIIHREKCASRNFGYGSNIKASMICAYAIGKDSCQGDSGGPLVVNNLLVGVVSWGIDCARPSYPGVYVDVSHVRSWIVSNAESI蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Specificity, limited to carboxyl side of arginine residue in B-chain of insulin.功能2:
/文献:
"Hypodermin A, a trypsin-like neutral proteinase from the insect Hypoderma lineatum." Tong N.T., Imhoff J.M., Lecroisey A., Keil B. Biochim. Biophys. Acta 658:209-219(1981)研究领域:
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文献和实验的作用是调控基因表达。例如组蛋白甲基化多导致基因沉默,去甲基化则相反;乙酰化一般是转录激活,去乙酰化则相反。当然,也可在此基础上产生复杂的生物学效应。例如组蛋白去乙酰化酶 HDAC 可影响免疫系统;H3K4me3、H3K9me2 能够调控记忆的形成, 而且 H3K 甲基化与 X 染色体失活、基因组印记和异染色质形成有关;H3 乙酰化通过多种机制调控以来 ATP 的染色质重塑 ,并参与炎症反应;H2A、H2B 泛素化则与 DNA 损害反应有关;而 H3S28 磷酸化与 H3K27 乙酰化可激活转录
含有的去污剂,树脂能够从蛋白水溶液和肽样本中去除自由的,未结合的阴离子的,非离子或两性离子的去污剂 (e.g. SDS, Triton® X-100 或 CHAPS),同时对于下游进行的质谱或其他技术的分析应用带来最小的样本丢失。 DetergentOUT™ GB-S10 柱子在第三方的研究使用中表明完全兼容 DI-QTOF 和 LC-MS/MS(请见参考文献)。DetergentOUT™ GB-S10 柱子的使用显著增加到了检测到的肽光谱的数量。除此之外,DetergentOUT™ GB
杆菌的包涵体可采用离心收获。 洗涤 包涵体常用的洗涤试剂为中性去垢剂和还原剂。包涵体中主要由重组蛋白组成,同时也含有一些外膜蛋白(Omp C、Omp F、Omp A)、16S 和 23S、5S的r RNA、质粒 DNA 和其它杂质。去除这些杂质,可采用去垢剂 Triton X-100、脱氧胆酸盐和低浓度的变性剂等对包涵体充分洗涤。包涵体洗涤的洁净程度,对于后续的纯化步骤十分重要,包涵体纯度高,可以大大降低后续的纯化压力。但是,变性剂尿素的使用应该注意的是大肠杆菌外膜蛋白Omp T(37 kD
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