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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Vaccinia virus Protein A33 (A33R), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4956.0 |
| 规格: | 20μg | 产品价格: | ¥2616.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
A33RUniprot No.:
P68616别名:
A33RProtein A33种属:
Vaccinia virus (strain Copenhagen) (VACV)蛋白长度:
Partial来源:
Yeast分子量:
16.2 kDa表达区域:
57-185aa氨基酸序列:
VRLNQCMSANEAAITDAAVAVAAASSTHRKVASSTTQYDHKESCNGLYYQGSCYILHSDYQLFSDAKANCTAESSTLPNKSDVLITWLIDYVEDTWGSDGNPITKTTSDYQDSDVSQEVRKYFCVKTMN蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Coordinates the incorporation of A36 into intracellular enveloped virion membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990)Function:
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文献和实验High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris
. Here we describe a large-scale fed-batch fermentation process with an efficient online closed-loop methanol control for the production of the recombinant protein. Purification of A33scFv from clarified culture medium was done using a two-step chromatographic procedure
变化。对于肠癌研究来说,人肠上皮细胞表面抗原基因 A33 启动子,可作为组织特异性启动子使用。按例祭出神图一张,定制你的专属载体,就是这么简单! 上海吉凯基因化学技术有限公司成立于 2002 年,拥有 BSL-2 级别的慢病毒包装实验室。病毒生产线通过 ISO9001 质量管理体系验证,月均定制基因病毒产品包装超过 1000 次。慢病毒生产采用 6 项 QC 检测、病毒纯度分级纯化以及病毒滴度绝对定量检测确保病毒质量。 400 名兢兢业业的吉凯人,用专业换您的高效。参考文献【1】Ahmed, D
cDNA Expression Cloning in Mammalian Cells
Fuerst, T.R., Niles, E.G., Studier, F.W., and Moss, B. 1986. Eukaryotic transient‐expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 83:8122‐8126
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