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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Cadherin-17 (Cdh17), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Cadherin-17 (Cdh17), partial品名简称:
Recombinant Mouse Cdh17 protein, partial货号:
CSB-EP882608MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Cdh17Uniprot No.:
Q9R100别名:
BILL-cadherin (Liver-intestine cadherin) (LI-cadherin) (P130)种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
13.1 kDa表达区域:
173-253aa氨基酸序列:
INDVMYFQIDSKTGAISLTPEGSQELDPVKNPSYNLVVSVKDMGGQSENSFSDTTYVDISIRENIWKAPEPVEIRENSTDP蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine.功能2:
/文献:
"Mechanisms involved in vitamin D mediated intestinal calcium absorption and in non-classical actions of vitamin D." Christakos S., Dhawan P., Ajibade D., Benn B.S., Feng J., Joshi S.S. J. Steroid Biochem. Mol. Biol. 121:183-187(2010)研究领域:
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文献和实验Cadherin-Mediated Cell-Cell Interactions
, Drosophila Epithelia, skin, osteoclasts, liver, pancreas, kidney, gonads, placenta brain, sensory neurons N-Cadherin (ACAM) Human, mouse, bovine, chicken
(truncated cadherin) (CDH13). - Muscle (M-cadherin) (CDH14). - Liver-intestine (LI-cadherin). - EP-cadherin.
多篇 SCI 发现用 RIPA 提蛋白存在问题,我们该如何应对?
90, c-Src, 和 tubulin,表明这些蛋白在 RIPA 不可溶组分中大量存在(下图)。Wang 等 [6] 对比 SDS 加热法和 RIPA 裂解液法从斑马鱼肝脏肿瘤中提取蛋白,发现 RIPA 裂解液提取这些样品时对于大分子量蛋白的提取效率十分低(下图)。Li[7] 对比了从小鼠脾脏和肝脏中提取总蛋白 RIPA 可溶和不可溶性组分以及基于离心管柱法商业试剂盒提取的蛋白质图谱,发现 RIPA 不可溶组分蛋白质图谱与可溶性组分图谱相似,但是不完全相同。这些丢失的不可溶组分覆盖了整个蛋白质图谱分子
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