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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human PITH domain-containing protein 1 (PITHD1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human PITH domain-containing protein 1 (PITHD1)品名简称:
Recombinant Human PITHD1 protein货号:
CSB-EP867109HU规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PITHD1Uniprot No.:
Q9GZP4别名:
C1orf128种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
31.6 kDa表达区域:
1-211aa氨基酸序列:
MSHGHSHGGGGCRCAAEREEPPEQRGLAYGLYLRIDLERLQCLNESREGSGRGVFKPWEERTDRSKFVESDADEELLFNIPFTGNVKLKGIIIMGEDDDSHPSEMRLYKNIPQMSFDDTEREPDQTFSLNRDLTGELEYATKISRFSNVYHLSIHISKNFGADTTKVFYIGLRGEWTELRRHEVTICNYEASANPADHRVHQVTPQTHFIS蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004)研究领域:
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文献和实验【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
也可以从其他物种中去除白蛋白。适用于处理 25 或 50 个样本。Figure 1: 2D analysis of whole human serum before (left) and after(right) treatment with AlbminOUT™. 产品特点: ● 从样本中不到 10 分钟的时间内去除白蛋白 ● 基于白蛋白和 Cibachron 蓝色染料的结合 ● 每个柱子的结合力大于 2 mg 的人白蛋白 ● 从 5-50
Nat Biotechnol:调控蛋白质稳态,建立蛋白降解靶向减毒疫苗新策略
,因此 PROTAC 病毒在疫苗制备细胞中可以高效复制而大量制备。 图 1.PROTAC 病毒疫苗原理。VP, 病毒蛋白 (viral protein);Ub, 泛素(ubiquitin);PTD, 蛋白降解靶向元件(proteolysis-targeting domain) 根据上述设计原理,该团队首先构建了一株 PROTAC 流感病毒疫苗,命名为 M1-PTD。对病毒生长曲线考察发现,M1-PTD 只能在 PROTAC 病毒制备细胞中高效复制而得以制备,而在正常细胞中复制能力显著下降而安全(图
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