///蛋白Recombinant Lecanicillium

纯度：

Greater than 85% as determined by SDS-PAGE.

基因名：

/

Uniprot No.：

Q68GV9

别名：

Alkaline serine protease ver112; EC 3.4.21.-

种属：

Lecanicillium psalliotae (Verticillium psalliotae)

蛋白长度：

Full Length of Mature Protein

来源：

E.coli

分子量：

36.0 kDa

表达区域：

103-382aa

氨基酸序列：

AITQQQGATWGLTRISHRARGSTAYAYDTSAGAGACVYVIDTGVEDTHPDFEGRAKQIKSYASTARDGHGHGTHCAGTIGSKTWGVAKKVSIFGVKVLDDSGSGSLSNIVAGMDFVASDRQSRNCPRRTVASMSLGGGYSAALNQAAARLQSSGVFVAVAAGNDNRDAANTSPASEPTVCTVGATDSNDVRSTFSNYGRVVDIFAPGTSITSTWIGGRTNTISGTSMATPHIAGLAAYLFGLEGGSAGAMCGRIQTLSTKNVLTSIPSGTVNYLAFNGAT

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

13-23 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Serine protease which can degrade the nematode cuticle.

内毒素：

Not test.

SDS-PAGE:

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP737896LCAT could indicate that this peptide derived from E.coli-expressed Lecanicillium psalliotae (Verticillium psalliotae) /.

LC-MS Image Description/Western Blot:

/

Product types:

Developed Protein

Biological_Activity:

/

Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes." Liang L., Meng Z., Ye F., Yang J., Liu S., Sun Y., Guo Y., Mi Q., Huang X., Zou C., Rao Z., Lou Z., Zhang K.Q. FASEB J. 24:1391-1400(2010)

Function:

Serine protease which can degrade the nematode cuticle.