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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli RNA polymerase sigma factor rpoS (rpoS)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥5370.0 |
| 规格: | 20μg | 产品价格: | ¥3324.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
rpoSUniprot No.:
P13445别名:
Sigma S Sigma-38种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
E.coli分子量:
38.1 kDa表达区域:
1-330aa氨基酸序列:
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE蛋白标签:
Tag-Free产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
TranscriptionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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- 作者
- 内容
- 询问日期
文献和实验Extracting Regulatory Networks of Escherichia coli from RegulonDB
, including sigma–gene relationships corresponding to transcription of alternative RNA polymerase holoenzyme promoters; as well as, transcription factor (TF)-genes, TF–operons, TF–TF, and TF–regulon interactions. 17
Cold-Inducible Promoters for Heterologous Protein Expression
Rapid transfer of exponentially growing E. coli cultures from physiological to low temperatures (10–15#X00B0;C) has profound consequences on cell physiology: membrane fluidity decreases, which interferes with transport and secretion
Assays for Transcription Factor Activity
modification, and, when bound at target promoters, they can stimulate the initiation of transcription by RNA polymerase (1 ). This chapter is concerned with in vitro methods for measuring the transcription activation function of this important class
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