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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Otopetrin-1 (Otop1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Otopetrin-1 (Otop1)品名简称:
Recombinant Mouse Otop1 protein货号:
CSB-CF772148MO(A4)规格:
20μg价格:
9720LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Otop1Uniprot No.:
Q80VM9别名:
Otopetrin-1种属:
Mus musculus (Mouse)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
68.6 kDa表达区域:
1-600aa氨基酸序列:
MPGGPGAPSSPAASSGSSRAAPSGIAACPLSPPPLARGSPQASGPRRGASVPQKLAETLSSQYGLNVFVAGLLFLLAWAVHATGVGKSDLLCVLTALMLLQLLWMLWYVGRSYMQRRLIRPKDTHAGARWLRGSITLFAFITVVLGCLKVAYFIGFSECLSATEGVFPVTHAVHTLLQVYFLWGHAKDIIMSFKTLERFGVIHSVFTNLLLWANSVLNESKHQLNEHKERLITLGFGNITIVLDDHTPQCNCTPPALCSALSHGIYYLYPFNIEYQILASTMLYVLWKNIGRRVDSSQHQKMQCRFDGVLVGSVLGLTVLAATIAVVVVYMIHIGRSKSKSESALIMFYLYAITVLLLMGAAGLVGSWIYRVDEKSLDESKNPARKLDVDLLVATGSGSWLLSWGSILAIACAETRPPYTWYNLPYSVLVIVEKYVQNIFIIESVHLEPEGVPEDVRTLRVVTVCSSEAAALAASTLGSQGMAQDGSPAVNGNLCLQQRCGKEDQESGWEGATGTTRCLDFLQGGMKRRLLRNITAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAAALFEVYCKI蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
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文献和实验071794 h 货号:CSB-RP071794(A4/C) 兔抗人淀粉样肽 beta A4 蛋白多克隆抗体 货号:CSB-PA07179A0Rb 识别种属:人,小鼠 WB:Mouse heart tissue,Mouse kidney tissue,Mouse lung tissue,293,A549,U251,SH-SY5Y IHC:human kidney
实验助攻 | WB 实验 (Western Blot) 杂带太多?原因就这几个!
发生降解时,完整的蛋白分子会被蛋白酶切断。如果降解发生在抗体结合域,或者不同分子被切割的位置不同,就会产生分子量比原目的蛋白小的一系列截短片段。此时,WB 条带中不仅会有原本的主带,还会出现多条低分子量的杂带或条带模糊、向下拖尾的现象。 Tips 如果使用纯化的重组蛋白作为阳性对照只有单条带,而你的实验样本出现多条带,则降解可能性极大。 【建议】 ① 添加抑制剂:裂解液确保含有蛋白酶抑制剂,并超声,抑制内源性蛋白酶的活性。 ② 低温操作:样本收集、处理和裂解的全过程必须在冰上进行,并使用
哺乳动物细胞和悬浮哺乳动物细胞的培养 ◆疫苗生产中病毒颗粒的制备 ◆支持杂交瘤形成时的细胞融合 ◆支持单核细胞、巨噬细胞、上皮细胞和成纤维细胞系的细胞生长 适应的细胞类型 Cell lines and Cell types ● 235-1,MNQ Rat Pituitary 大鼠垂体脑细胞 ● B82 Mouse L cells –connective tissue 小鼠L细胞联结组织 ● COS1, COS
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