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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Troponin T, cardiac muscle (Tnnt2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Troponin T, cardiac muscle (Tnnt2)品名简称:
Recombinant Rat Tnnt2 protein货号:
CSB-EP341903RA规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Tnnt2Uniprot No.:
P50753别名:
Cardiac muscle troponin T种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
41.6 kDa表达区域:
2-299aa氨基酸序列:
SDAEEEVVEYEEEQEEEDWSEEEEDEQEEAVEEEDGEAEPDPEGEAEAEEDKAEEVGPDEEARDAEDGPVEDSKPKPSRLFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELISLKDRIEKRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.功能2:
/文献:
/研究领域:
Biochemicals请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and SELDI-TOF
Cardiac troponin I (cTnI) is a key regulator of cardiac muscle contraction. Upon myocardial cell injury, cTnI is lost from the cardiac myocyte and can be detected in serum, in some cases with specific disease-induced modifications, making
Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes
in myocytes now makes it possible to investigate the relative phosphorylation of a given contractile protein as it resides within the intact myofilament. Contractile proteins, including troponin I (TnI), troponin T (TnT), myosin light chain 2 (MLC
心肌肌钙蛋白T启动子适合心肌靶向吗?AAV9动物验证数据解析
心肌肌钙蛋白T启动子(cardiac troponin T promoter)是一类用于心肌靶向表达的组织特异性启动子。吉凯基因内部动物验证显示,AAV9搭载心肌肌钙蛋白T启动子的EFP载体经尾静脉递送后,可在心肌区域获得明显增强的荧光信号。 1. 为什么心血管研究要关注组织特异性启动子? 启动子会影响: 目的基因在哪里表达 表达强度 非目标组织是否出现背景信号 结果是否容易解释 传统强启动子如CMV可获得较高表达,但可能存在非目标组织表达、背景信号较高、组织特异性不足等问题。 2. 心肌肌钙蛋白
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