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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)品名简称:
Recombinant E.coli aroH protein货号:
CSB-EP355572ENV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
aroHUniprot No.:
P00887别名:
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
E.coli分子量:
42.8 kDa表达区域:
1-348aa氨基酸序列:
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTERLVEKLASAVDTRF蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).功能2:
/文献:
"Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli." Zurawski G., Gunsalus R.P., Brown K.D., Yanofsky C. J. Mol. Biol. 145:47-73(1981)研究领域:
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文献和实验Measurement of NO and NO Synthase
Schmidt, H.H., Hofmann, H., Schindler, U., Shutenko, Z.S., Cunningham, D.D., and Feelisch, M. 1997. No NO from NO synthase. Proc. Natl. Acad. Sci. U.S.A. 93:14492‐14497.
氨甲酰磷酸合酶 carbamoyl phosphate synthase
催化由 NH3 、 CO2 与 2分子的 ATP合成氨甲酰磷酸的不可逆反应的酶, EC2. 7. 2. 5。存在于哺乳类的肝脏、蛙的肝脏、大肠杆菌等,分子量 31.5万(蛙肝)。对于动物酶的活性, N-乙酰谷氨酸是必需的,经此酶生成的氨甲酰磷酸是生成精氨酸、尿素或合成嘧啶的素材。大肠杆菌的酶亦能利用谷酰胺作为氨基供体。此外在蕈类,如伞菌( Agaricus),存在着只利用谷酰胺作为氨基供体的酶( EC2. 7. 2. 9)。
Purification of the Constitutive Nitric Oxide Synthase
Constitutive nitric oxide synthase (NOS) exists as two isoforms: endothelial (e) and neuronal (n) (reviewed in ref. 1 ). The first reports on the purification of eNOS and nNOS were by Schmidt et al. (2 ) and Bredt and Snyder
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