UGT78D1/UGT78D1蛋白Recombinant A

纯度：

Greater than 85% as determined by SDS-PAGE.

基因名：

UGT78D1

Uniprot No.：

Q9S9P6

别名：

Flavonol-3-O-glucoside L-rhamnosyltransferase (UDP-rhamnose:flavonol 3-O-glucoside rhamnosyltransferase)

种属：

Arabidopsis thaliana (Mouse-ear cress)

蛋白长度：

Full Length

来源：

Yeast

分子量：

52.6 kDa

表达区域：

1-453aa

氨基酸序列：

MTKFSEPIRDSHVAVLAFFPVGAHAGPLLAVTRRLAAASPSTIFSFFNTARSNASLFSSDHPENIKVHDVSDGVPEGTMLGNPLEMVELFLEAAPRIFRSEIAAAEIEVGKKVTCMLTDAFFWFAADIAAELNATWVAFWAGGANSLCAHLYTDLIRETIGLKDVSMEETLGFIPGMENYRVKDIPEEVVFEDLDSVFPKALYQMSLALPRASAVFISSFEELEPTLNYNLRSKLKRFLNIAPLTLLSSTSEKEMRDPHGCFAWMGKRSAASVAYISFGTVMEPPPEELVAIAQGLESSKVPFVWSLKEKNMVHLPKGFLDRTREQGIVVPWAPQVELLKHEAMGVNVTHCGWNSVLESVSAGVPMIGRPILADNRLNGRAVEVVWKVGVMMDNGVFTKEGFEKCLNDVFVHDDGKTMKANAKKLKEKLQEDFSMKG_S_SLENFKILLDEIVKV

蛋白标签：

N-terminal 10xHis-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

25-35 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Acts as a UDP-rhamnose:flavonol-3-O-glucoside rhamnosyltransferase. Keampferol and quercitin are used as substrates. Possesses low quercetin 3-O-glucosyltransferase activity in vitro.

内毒素：

Not test.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

/

Product types:

Developed Protein

Biological_Activity:

/

Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"UGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana." Jones P., Messner B., Nakajima J., Schaffner A.R., Saito K. J. Biol. Chem. 278:43910-43918(2003)

Function:

Acts as a UDP-rhamnose