PROK/PROK蛋白Proteinase K Recomb

纯度：

Greater than 95% as determined by SDS-PAGE.

基因名：

PROK

Uniprot No.：

P06873

别名：

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种属：

Tritirachium album (Engyodontium album)

蛋白长度：

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来源：

Yeast

分子量：

28.9 kDa

表达区域：

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氨基酸序列：

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蛋白标签：

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产品提供形式：

Liquid or Lyophilized powder

缓冲液：

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货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

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内毒素：

Not test.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. According to the left SDS-PAGE image, the purity of Proteinase K is 95%+ and the molecular weight is 28.9 kDa.

LC-MS Image Description/Western Blot:

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Product types:

Active Protein & In Stock Protein

Biological_Activity:

①Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated ＞30U/mg. Unit definition:One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C ②Detect Nucleic acid residue by agarose gel electrophores. ③Detect DNase residue by agarose gel electrophores. ④Detect RNase residue by agarose gel electrophores. ⑤The Proteinase K powder was stored at 4℃. And its enzyme activity was assayed every month over the past year. The left chart shows how its enzyme activity changes in a year. It turns out the enzyme activity is maintained more than 95%, which indicates that proteinase K is very stable.

Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

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Function:

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