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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Granulocyte-macrophage colony-stimulating factor (Csf2) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10μg
英文名称:
Recombinant Mouse Granulocyte-macrophage colony-stimulating factor (Csf2) (Active)品名简称:
Recombinant Mouse Csf2 protein (Active)货号:
CSB-AP003661MO规格:
10μg价格:
1740LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
The ED50 as determined in a cell proliferation assay using PDC-P1 cells is 15-50 pg/ml.基因名:
Csf2Uniprot No.:
P01587别名:
Granulocyte-macrophage colony-stimulating factor;Csf2;GM-CSF;Colony-stimulating factor;Csfgm种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
14.2 kDa表达区域:
18-141aa氨基酸序列:
APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPGQK蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.功能2:
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.文献:
/研究领域:
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文献和实验Acute myeloid leukemia (AML) has an annual incidence of 2.4 cases per 100,000 persons and is the most common form of acute leukemia in adults (1 , 2 ). AML is a model for drug-resistant human cancers, and current therapies for AML possess
碱性粒细胞的增殖,阻止肥大细胞发生程序性细胞死亡。 (二)GM-CSF 1977年Burgess等从小鼠肺条件培养液中发现一种能刺激粒细胞和巨噬细胞形成集落的因子,命名为粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)。1984年和1985年小鼠和人GM-CSF的cDNA分别克隆成功。 1.GM-CSF的产生 T细胞、B细胞、巨噬细胞、肥大细胞、内皮细胞、成纤维细胞等均可产生GM-CSF
【进展|热点】BLOOD: 雌激素受体通过上调IRF4促进DC的分化
Estrogen receptor signaling promotes dendritic cell differentiation by increasing expression of the transcription factor IRF4 Blood, 14 January 2010, Vol. 115, No. 2, pp. 238-246. During inflammation, elevated granulocyte macrophage–colony
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