All cytochrome P450 assays were conducted at 0.5 mg/ml protein with an NADPH generating system (1.3 mM NADP, 3.3 mM glucose 6-phosphate and 0.4 U/ml glucose 6-phosphate dehydrogenase) and 3.3 mM MgCl2,0.2 mM phenacetin (delivered as a MeOH solution, final concentration = 1% MeOH), 0.2 mM testosterone (delivered as an acetonitrile solution, final concentration = 1%acetonitrile) ,0.2mM Dextromethorphan (delivered as a MeOH solution, final concentration = 1% MeOH), 0.2mM Coumarin(delivered as a MeOH solution, final concentration = 1% MeOH), 2mM Chlorzoxazone (delivered as an acetonitrile solution, final concentration = 1%acetonitrile)in 100 mM potassium phosphate (pH 7.4) was incubated at 37°C for 30 and 60 minutes. Caution:Treat all products containing human and monkey-derived materials a potentially infectious, as no known test methods can offer assurance that products derived from human or monkey tissues will not transmit infectious agents. All products are for research use only. Do not use in animals or humans. These products have not been approved for any diagnostic or clinical procedures.