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文献和实验of flies containing the homozygous human UAS wild-type α-synuclein transgene was shown by specific immunological blotting procedures. Behavioral testing indicated that motor rather than sensory functions were principally affected by the presence of α
, for which there is either a wild-type allele (GTT) or deletion. Using MassARRAY® software, assays can easily be designed so that there is a clear distinction between the mutations and wild type. In this particular assay, the mass for the deletion is at 7764 Da, and the mass
and sodium pyruvate (1 mM) and hepatoma (HuH7) cells were cultured in DMEM + GlutaMAX. Both media contained 10% FBS. All cell culture reagents were purchased from Invitrogen (GIBCO®). Roferon (Interferon alpha2a, ROCHE) was diluted in fresh medium to a final
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