Anti-mouse IgG HRP-linked Anti

Conjugation of Antibody to HRP

of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator.  Desalt the HRP on PBS equilibrated column.  Collect

人抗风疹病毒IgG抗体（anti-RV IgG）酶联免疫分析（ELISA）

中依次加入抗风疹病毒 IgG 抗体（ anti-RV IgG ），再与 HRP 标记的抗原结合，形成抗原 - 抗体 - 酶标抗原复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体（ anti-RV IgG ）呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中人抗风疹病毒 IgG 抗体（ anti-RV IgG ）浓度。 试剂盒组成

Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays

criteria must be met. Laboratory testing to identify aPL antibodies includes lupus anticoagulant (liquid-based clotting assays) and immunological solid-phase assays (usually enzyme-linked immunosorbent assay formats) for IgG and/or IgM anticardiolipin (aCL