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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
Methyl 2-(4-formylphenyl)acetate
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
96524-70-8
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验You will need for 1° cDNA synthesis: 5 x SuperScript RTase buffer (Gibco-BRL) 5mM methyl dNTPs (Pharmacia, substitute 5m dCTP for dCTP) 100mM DTT (Gibco-BRL) oligo dT primer/adaptor [a-32P] dATP (~400Ci/mmol, Amersham or DuPont) Ribonuclease inhibitor
tube mix: 15 µl dH2O (times number of samples + 1) 70 µl 1MTris Ph 8.0 (times number of samples + 1) 20 µl 4 mM Acetyl CoA (times number of samples + 1) and 5 µl 14 C CAM (times number of samples). 13. Add 0.5 ml Ethyl Acetate to each tube. 14
in 30 µl Ethyl Acetate. 19. Spot on silica gel TLC (in 5 µl amounts). 20. Develop TLC in 200 ml Chloroform/Methyl-OH (95:5) until solution is almost at the top (app. 1 hr.). 21. Dry & expose against film ON.




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