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- 文献和实验
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- 英文名:
Octaethylene Glycol Mo...
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- 供应商:
无锡云萃生物
- CAS号:
25990-96-9
- 规格:
2mg/10mg/500mg/638g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 638g | 产品价格: | ¥238.0 |
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文献和实验v468 Chapter 9 自身启动子报告基因验证转录靶点
,Ontario, Canada). 5. 2.5 U/μL Pfu DNA polymerase (e.g. from Fermentas), suppliedwith appropriate 10× reaction buffer. 6. 96% Tetramethylene sulfoxide (TMSO) (e.g. from Sigma,St. Louis, MO). 7. Qiaquick PCR Purification Kit (Qiagen) or similar kit
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
of a reporter gene also requires consideration. In order to see all the progeny of infected cells, a constitutive promoter should be used. We have had success using the LTR of Moloney Murine Leukemia Virus (Mo-MLV) for work in rats and mice and the LTR of Avian
) for 1 hour, or bake in an 80・ oven for about 15 min. Once dried, you can store the pads by sticking them back in the original coverslip box. b) 10X microinjection buffer . 20 % polyethylene glycol, molecular weight 6000-8000 200 mM potassium










