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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
9Z,12E-Tetradecadienyl...
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
30507-70-1
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验of the pellet, transfer cell in 15 ml Falcon tube Repeat steps 9 - 12, add just 10 ml fixative Remove fixative until about 2 ml final volume Resuspend pellet and apply suspension on slides: Cool slides to -20°C (e.g. put about 10
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
A quick RNA mini-prep for Neurospora mycelial cultures
overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash pellet with 70 EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12.










