E484Q-新冠突变重组蛋白

SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His Recombinant Protein: 产品信息
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His(Cat:40592-V08H81), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His(Cat:40592-V08H81) is 5.0-25.0 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(E484Q)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(Arg319-Phe541(E484Q))-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa. As a result of glycosylation, it migrates as an approximately 35.1 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.