- ￥656
- Sigma-Aldrich
- 进口
- M33476-50G
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
2-Methylbutyraldehyde
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
96-17-3
- 规格：
50G
属性
质量水平
100
检测方案
95%
形式
liquid
折射率
n20/D 1.3919 (lit.)
bp
90-92 °C (lit.)
密度
0.806 g/mL at 20 °C
0.804 g/mL at 25 °C (lit.)
SMILES字符串
[H]C(=O)C(C)CC
InChI
1S/C5H10O/c1-3-5(2)4-6/h4-5H,3H2,1-2H3
应用
- 在乙酸铵存在的情况下通过与各种苯乙酮和丙二qing的多组分缩合反应合成的 2-氨基-3-氰基吡啶衍生物。
- 在氢分子存在的情况下通过与二yi胺的铑催化还原胺化反应合成的N,N-二乙基-2-甲基-1-丁胺。
- α-采用三环非离子强路易斯碱与苯yi腈进行缩合反应合成的（2-甲基亚丁基）苯yi腈。
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文献和实验Investigation of volatile metabolites during growth of Escherichia coli and Pseudomonas aeruginosa by needle trap-GC-MS.
A new method for the growth-dependent headspace analysis of bacterial cultures by needle trap (NT)-gas chromatography-mass spectrometry (GC-MS) was established. NTs were used for the first time as enrichment technique for volatile organic compounds (VOCs) in the headspace of laboratory cultures. Reference strains of Escherichia coli and Pseudomonas aeruginosa were grown in different liquid culture media for 48 h at 36 °C. In the course of growth, bacterial culture headspace was analysed by NT-GC-MS. In parallel, the abiotic release of volatile organic compounds (VOC) from nutrient media was investigated by the same method. By examination of microbial headspace samples in comparison with those of uninoculated media, it could be clearly differentiated between products and compounds which serve as substrates. Specific microbial metabolites were detected and quantified during the stationary growth phase. P. aeruginosa produced dimethyl sulfide (max. 125 μg L(-1) < limits of quantification (LOQ)), 1-undecene (max. 164 μg L(-1)) and 2-nonanone (max. 200 μg L(-1)), whereas E. coli produced carbon disulfide, butanal and indole (max. 149 mg L(-1)). Both organisms produced isoprene.
Sephadex G-50 spun column purificati
Submission Details: *Dr. Simon Dawson*Department of Biochemistry*University of Nottingham*U.K.*17:03*22/1/96. Database: Method/Protocol-->Spin column purification can be used to change buffers without a concomitant change in solution
后从第九第十孔中各取 50 μ l弃掉。（稀释后各孔加样量都为 50 μ l，浓度分别为 12 n g/L ，8 n g/L ，4 n g/L ，2 n g/L ，1 n g/L）。 2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、 待测样品孔 。在酶标包被板上待测样品孔中先加样品稀释液40 μ l，然后再加待测样品 10 μ l（样品最终稀释度为 5 倍）。 加样将样品加于酶标板孔底部，尽量不触及孔壁， 轻轻 晃动 混匀 。 3.
96 Well Mating Protocol platewise
: 1 liter Milli Q water 5 g yeast extract 10 g NaCl (5 g for any Zeocin recombinants) 2 g DL-p-Chlorophenylalanine powder (will not dissolve before autoclaving) 20 g agar AUTOCLAVE After media has cooled to 55℃ add the following and stir gently: 8 ml 50
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