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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Lecithin retinol acyltransferase protein (24-182AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
O95237
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
LRAT
- 抗体英文名:
LRAT Antibody, HRP conjugated
- 抗体名:
LRAT;Lecithin retinol acyltransferase;Phosphatidylcholine--retinol O-acyltransferase
- 规格:
100μl/50μl
| 规格: | 100μl | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Antibody Staining of Larval Imaginal Discs
4. Incubate with primary antibody in PBTN for 1 hr. to overnight. 5. Wash tissue for 30 min. to overnight in PBS. 6. Incubate for 1 hr. to overnight with HRP-conjugated secondary diluted 1:100 in PBTN. 7. Wash 45 min. with 3 changes
Whole mount antibody staining of zebrafish embryos for markers of segmentation
1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps. Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking at 4oC. 2. Wash with 5 ml 0.1% BSA in PBS for 10 minutes. Wash 3X with 5 ml PBS
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