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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
(Z)-ethyl 2-(piperidin-3-ylidene)acetate hydrochloride
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
957472-02-5
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验using the GS3 rotor. Repeat if necessary. 15b. Combine the DNA-containing supernatants and precipitate the DNA in 35 ml Corex glass tubes adding 2.5 volumes of cold 95% ethanol/acetate. 16b. Resuspend the dried DNA pellet in 2 ml of 10:0.1 TE
2O, heat to 95° C, and allow to cool to RT slowly. This gives a final concentration of 5 pmoles/µL and makes enough adapter for 60 ligations (see below). MseI-adapter = 5'-GACGATGAGTCCTGAG (MseI-oligo.1) TACTCAGGACTCAT-5' (MseI-oligo.2
AFLP: not only for fingerprinting, but for positional cloning
Preparation EcoRI-adapter = 5'-CTCGTAGACTGCGTACC (EcoRI-oligo.1) CTGACGCATGGTTAA-5' (EcoRI-oligo.2) - mix 1.7 µg EcoRI-oligo.1 and 1.5 µg EcoRI-oligo.2, 3 µl 10X One-Phor-All+,Q.S. to 60 µL w/ dH2O, heat to 95° C, and allow to cool to RT slowly










