小鼠抗PKM单克隆抗体

产品名称：小鼠抗PKM单克隆抗体
产品规格：25 ul/100 ul/200 ul
以下范例说明仅供参考，具体产品资料欢迎咨询客服免费索取。
兔抗SYN1多克隆抗体 
英文名    Anti-SYN1 rabbit polyclonal antibody
别名    SYNI; SYN1a; SYN1b
相关类别    一抗    储存    冷冻（-20℃）
宿主    Rabbit    反应种属    Human, Mouse, Rat
标记物    Unconjugate    克隆类型    rabbit polyclonal
抗原    SYN1
Background:    This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified
Applications:    ELISA, WB
Name of antibody:    SYN1
Immunogen:    Synthetic peptide of human SYN1
Full name:    synapsin I
Synonyms：    SYNI; SYN1a; SYN1b
SwissProt:    P17600
ELISA Recommended dilution:    2000-5000

Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane: Mouse brain tissue, Primary antibody: D261157(SYN1 Antibody) at dilution 1/900, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds
小鼠抗PKM单克隆抗体Western印迹膜抗体清除剂使用方法
1、 将印迹膜浸入 Western 印迹膜洗膜液中，然后放入杂交袋中。如果本产品中提供的杂交带赠品大小不合，则需要自备大小合适的杂交袋。
2、 加入 30-50 mL 本产品（具体加入的量根据杂交袋大小决定），然后热封口。
3、 在 50℃水浴中保温 30 分钟，每 10-15 分钟手动摇晃杂交袋一次。
4、 保温结束后小心取出杂交袋，剪掉一角，将本产品倒入塑料瓶中，4℃密封保存（本产品可以反复使用 3-5 次）。
5、 将印迹膜放入干净托盘中，加入足够 Western 印迹膜洗膜液（以覆盖住膜表面为准），在摇床上轻柔摇晃 3-5 分钟。如此重复三次。如果洗涤三次后印迹膜上还有残留的巯基乙醇味道，则应该继续清洗直到没有味道为止。
6、 将印迹膜浸入甲醇原液中 30 秒。
7、 取出后在滤纸上放置 15 分钟。
8、 立即用于后续的 Western 杂交或包在保鲜膜中在 4℃长期保存。