- ￥683.88
- Sigma
- 美国
- 10831-100ML
- 2026年01月01日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海益启生物科技有限公司
- 规格：
100ML
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文献和实验Purpose Materials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS 43 ml blood 12 ml RT Histopaque 107718 ml cold H2 O2 ml 10x PBSM199 for HUVECs: 1L powder pocket
Lymphocyte Transformation 淋巴细胞转染
Principle:Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering onto histopaque. During centrifugation, erythrocytes and granulocytes are aggregated by ficoll
Method: Removal of Yeast Contamination from Lymphoblast Cultures
5 ml histopaque into a 15 ml centrifuge tube. Carefully layer on top of histopaque 10 ml of contaminated culture (or concentrated cells/yeast resuspended in growth media). Centrifuge tube for 25 minutes at 2500 rpm
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