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- 详细信息
- 文献和实验
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上海益启生物科技有限公司
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
. Stop the reaction by adding 2 µl of stop mix. Purify probe with either one of the following kits: NucTrap® Probe Purification Push Columns (Stratagene #400701), for DNA- or RNA-probes 17 bp - 50 kb Qiaquick PCR
PCR product purification-Molecular Biology
are you centrifuging in those columns? Gel purification kit columns cannot go over 3 min., otherwise your DNA gets stuch in the agarose mesh, so don't spin too long!!! Let the DNA air-dry really long after the EtOH precipitation, try to avoid 95 celsius heating
Sauer:RNA Purification from E. coli
this voracious periplasmic RNase from ruining my stable RNAs during lysis. If you plan on working with RNA in E. coli, I recommend knocking at least this gene out. It has no known phenotype. Keeping in mind that with long chases, radiolabeled substrate pools
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