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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
Benzo[c][1,2,5]thiadiazol-5-ylmethanol
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
89795-51-7
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
volume, so adjust as necessary). Incubate overnight at 55 C. Day 4: Remove all parafilm from tubes. Inactivate Proteinase K for 10-15 minutes at 95 C in PCR machine or hot water bath. Remove oil by rolling samples and oil on parafilm and and pipette
loading dye to samples. Denature samples at 95°C for 3 minutes then place directly on ice. Load 3.5 µl sample on gel. Run at 55W until the xylene cyanol migrates to the bottom of the gel. Transfer gel to 3 mm Whatman paper and dry.





