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- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
6-Bromo-7-methoxy-1,1-dimethyl-3,4-dihydronaphthalen-2(1H)-one
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
1256578-99-0
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine Cross-references are to Schwarzacher & Heslop-Harrison 2000. Practical in situ Hybridization. Bios, Oxford. 216+xii pp . Available
In situ immunodetection of 5-methylc
+ 1% BSA for 20 min at RT 5) Incubate slides in monoclonal anti-5-mC (1:1000 dilution in PBS) for 1h at 37℃ in a humid chamber 6) Wash in PBST 2x5min 7) Incubate slides in Biotin anti-mouse IgG (1:500 dilution in PBST) for 1h at 37℃ in a humid chamber
Preparation of active proteins from inclusion bodies.
twice with TBS or PBS containing 1% triton X 100 followed by spinning at 30 000g for 30min at 4C. - The pellet (inclusion bodies) is the solubilized in 2 ml 50mM HEPES-NaOH pH7.5, 6M guanidine HCl, 25mM DTT and left for 1h at 4C. - Insoluble











