XBP-1s (E7M5C) Mouse mAb

Purification of mAb (IgG)

ddH2O and filter as before. pH=3.0.(11) Regeneration buffer (BioRad): BioRad Affi-gel regeneration buffer.2. Procedure(1) For Ammonium sulfate cutFilter Sup. in 1L Costar Filter using pre-filter.Add 50 mM Tris pH7.8 (50 ml of 1 M stock/Liter): 100

Purification of mAb (IgG)

  Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

T-Cell Activation Using mAb to CD3

One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse