Cleaved Caspase-7 (Asp198) Ant

Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan

in studies determining the contribution of matrix metalloproteinases (MMPs) and aggrecanases to aggrecanolysis. In particular, an antibody to the A374 RGSV N terminus was instrumental in the landmark discovery of the aggrecanases, ADAMTS-4 and ADAMTS-5

monoclonal antibody production fusion

), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC =  Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC

Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial Cytoplasm

. The below protocol provides the detailed description of MBP-scFv production in E. coli utilizing two expression systems: pMalc-TNN and pMalc-NHNN. Although the MBP tag does not disrupt the most of antibody activities, the MBP-TNN-scFv product can be cleaved by TEV