QUANTITY: 50µL BACKGROUND:The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. SPECIFICITY:Antibody can detect endogenous Vimentin protein. ISOTYPE: IgG1 APPLICATIONS: WB, IF, IHC Species Cross-Reactivity:H, R, M Recommended dilutions: WB: 1:1,000-2,000 IHC: 1:200-500 *NOTE: Optimal working dilutions must be determined by the end user. STORAGE/HANDING:1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.Store at -20°C. Do not aliquot the antibody. Alternative Names: FLJ36605 antibody, VIM antibody, VIME antibody Western Blot: Western blot analysis of 1) Hela, 2) Immunohistochemical analysis of paraffin- Mouse Brain Tissue , 3) Rat Brain embedded human breast caricnomausing tissue with TDY199 diluted at 1:2,000. Vimentin (TDY199)Mouse mAb diluted at 1:500.
IF analysis of Hela with TDY199(Left) and DAPI (Right) diluted at 1:100. For research use only; not for use as a diagnostic.
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