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FRTL 5; FRTL5; FRTL-5 Cl 2
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充足
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Fischer
甲状腺
大鼠甲状腺细胞
大鼠
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上皮细胞样
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甲状腺
复苏发货或干冰冷冻发货
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粘附,具有凸起中心的团块
1 x 10^6 cells/vial/T25
细胞名称:FRTL-5大鼠甲状腺细胞
产品货号:C3104
描述:FRTL-5 细胞源自5至6周龄 Fischer 大鼠的甲状腺,与其他细胞系不同,FRTL-5 细胞倾向于以复杂的三维结构生长,而不是形成单层,为研究甲状腺细胞行为提供了独特的模型。大鼠品系:Fischer。
培养条件:推荐WHELAB自配培养基:
※ Leibovitz's L-15(货号:M0800)+ 10%胎牛血清
※ 配套完全培养基:(货号:M0801)
温度:37℃
气相:100%空气
生长特性:粘附,具有凸起中心的团块
细胞形态:上皮细胞样
产品规格:1 x 10^6 cells/vial/T25
冻存条件:细胞保存液(货号:G0200)
保存条件:液氮存储
用户须知:
1、关于货期:除复苏细胞外,承诺货期1周内到(节假日顺延),大多数产品1-3天内发货。细胞产品因需要复苏后进行1-2代传代调整后等待细胞长满,期间进行微生物检测,货期1、2周左右。
2、关于订购:为了保证您的权益,下单前务必联系我司客户服务热线(021-55150076)。
细胞传代:
1.弃去培养液,用PBS洗涤1-2次;
2.加入1.0ml胰酶消化液,37℃消化,显微镜下观察细胞消化情况,若细胞回缩变圆、透亮、轻拍甁壁呈流沙样脱落,则迅速拿回操作台,加入完全培养液,终止消化并轻轻吹打细胞,使其变成单细胞悬液;
3.将细胞收集于离心管中离心1000rmp/5min,弃上清,轻弹管底,将细胞弹散;
4.加入新鲜培养液重悬细胞,进行传代、冻存;
5.如果没有特别说明,建议收到细胞后的第一次传代比例为1:2。
6.注意培养基PH值变化,定期换液(每周2-3次)。
安全性:
所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。
联系人:胡经理
电话:15921028707(微信同号)
:3407506161
:021-55150076
官网:https://whelab.com
公司邮箱:info@whelab.com
技术邮箱:tech@whelab.com
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PubMed=6327870; DOI=10.1677/joe.0.1010269
Bidey S.P., Chiovato L., Day A., Turmaine M., Gould R.P., Ekins R.P., Marshall N.J.
Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro bioassay system for thyrotrophin.
J. Endocrinol. 101:269-276(1984)
Patent=US4608341
Ambesi-Impiombato F.S.
Living, fast-growing thyroid cell strain, FRTL-5.
Patent number US4608341, 26-Aug-1986
PubMed=2868604; DOI=10.1530/acta.0.1110054
Brandi M.L., Rotella C.M., Zonefrati R., Toccafondi R., Aloj S.M.
Loss of adrenergic regulation of cAMP production in the FRTL-5 cell line.
Acta Endocrinol. 111:54-61(1986)
PubMed=3475907; DOI=10.1530/acta.0.114s242
Ambesi-Impiombato F.S., Villone G.
The FRTL-5 thyroid cell strain as a model for studies on thyroid cell growth.
Acta Endocrinol. 116 Suppl. 281:242-245(1987)
PubMed=3670314; DOI=10.1128/mcb.7.9.3365-3370.1987
Fusco A., Berlingieri M.T., Di Fiore P.P., Portella G., Grieco M., Vecchio G.
One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes.
Mol. Cell. Biol. 7:3365-3370(1987)
PubMed=2545492; DOI=10.1016/0303-7207(89)90020-8
Sugawa H., Mori T., Imura H.
Establishment of 8-azaguanine-resistant mutants from rat thyroid cell line FRTL-5.
Mol. Cell. Endocrinol. 62:319-326(1989)
PubMed=2551628; DOI=10.1210/endo-125-4-1783
Pang X.-P., Hershman J.M., Chung M., Pekary A.E.
Characterization of tumor necrosis factor-alpha receptors in human and rat thyroid cells and regulation of the receptors by thyrotropin.
Endocrinology 125:1783-1788(1989)
PubMed=1694496; DOI=10.1210/endo-127-1-419
Ossendorp F.A., Bruning P.F., Schuuring E.M.D., van den Brink J.A.M., van der Heide D., de Vijlder J.J.M., de Bruin T.W.A.
Thyrotropin dependent and independent thyroid cell lines selected from FRTL-5 derived tumors grown in nude mice.
Endocrinology 127:419-430(1990)
CLPUB00429
Gerber H., Peter H.-J., Bosiger J., Studer H., Drews R., Ferguson D.C., Peterson M.E., Becker D.V.
Different continuous cell lines from adenomatous feline goiters widely differ in morphologic, functional and growth parameters.
(In) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon A., Gross J., Hennemann G. (eds.); pp.541-544; A A Balkema Publishers; Rotterdam (1991)
CLPUB00430
Peter H.-J., Gerber H., Studer H., Groscurth P., Zakarija M.
Thyroid cell lines forming follicle-like lumina in vitro.
(In) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon A., Gross J., Hennemann G. (eds.); pp.579-582; A A Balkema Publishers; Rotterdam (1991)
PubMed=1726925
Gerber H., Peter H.-J., Asmis L., Studer H.
Thyroid cell lines in research on goitrogenesis.
Thyroidology 3:115-118(1991)
PubMed=1846098; DOI=10.1210/endo-128-1-211
Peter H.-J., Gerber H., Studer H., Groscurth P., Zakarija M.
Comparison of FRTL-5 cell growth in vitro with that of xenotransplanted cells and the thyroid of the recipient mouse.
Endocrinology 128:211-219(1991)
PubMed=7836899; DOI=10.1677/joe.0.1430527
MacNeil S., Munro D.S., Metcalfe R., Cotterell S., Ruban L., Davies R., Weetman A.P.
An investigation of the ability of TSH and Graves' immunoglobulin G to increase intracellular calcium in human thyroid cells, rat FRTL-5 thyroid cells and eukaryotic cells transfected with the human TSH receptor.
J. Endocrinol. 143:527-540(1994)
PubMed=7912172; DOI=10.1111/j.1365-2265.1994.tb03017.x
Michelangeli V.P., Munro D.S., Poon C.W., Frauman A.G., Colman P.G.
Measurement of thyroid stimulating immunoglobulins in a new cell line transfected with a functional human TSH receptor (JPO9 cells), compared with an assay using FRTL-5 cells.
Clin. Endocrinol. (Oxf.) 40:645-652(1994)
PubMed=8761378; DOI=10.1038/bjc.1996.409
van der Kallen C.J.H., Coes J.H., van Grafhorst J.P., Schuuring E.M.D., Ossendorp F.A., Thijssen J.H.H., Blankenstein M.A., de Bruin T.W.A.
Dissociation of thyrotropin receptor function and thyrotropin dependency in rat thyroid tumour cell lines derived from FRTL-5.
Br. J. Cancer 74:606-612(1996)
PubMed=9709917; DOI=10.1089/thy.1998.8.623
Tasevski V., Benn D., Peters G., Luttrell B., Simpson A.
The Fischer rat thyroid cell line FRTL-5 exhibits a nondiploid karyotype.
Thyroid 8:623-626(1998)
PubMed=20151827; DOI=10.1089/thy.2009.0171
Sebai H., Hovsepian S., Ristorcelli E., Aouani A., Lombardo D., Fayet G.
Resveratrol increases iodide trapping in the rat thyroid cell line FRTL-5.
Thyroid 20:195-203(2010)
PubMed=21087161; DOI=10.1089/ast.2010.0461
Albi E., Ambesi-Impiombato F.S., Villani M., De Pol I., Spelat R., Lazzarini R., Perrella G.
Thyroid cell growth: sphingomyelin metabolism as non-invasive marker for cell damage acquired during spaceflight.
Astrobiology 10:811-820(2010)
DOI=10.1128/mcb.00663-17
Fusco A., Berlingieri M.T., Di Fiore P.P., Portella G., Grieco M., Vecchio G.
Retraction for Fusco et al., 'One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes'.
Mol. Cell. Biol. 38:e00663.17.1-e00663.17.1(2018)
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C3104 FRTL-5.pdf 附 (下载 2 次)