手机验证
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RAMOS; Ramos 1; RA 1; RA.1; Ra #1; Ra No. 1; Ramos(RA1); Ramos-RA1; Ramos (RA 1); Ramos (RA)
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充足
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腹水
人B淋巴细胞瘤细胞
人
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淋巴母细胞
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腹水
复苏发货或干冰冷冻发货
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悬浮
1 x 10^6 cells/vial/T25
细胞名称:Ramos人B淋巴细胞瘤细胞 (STR鉴定正确)
产品货号:C1459
培养条件:1640+20%FBS(配套完全培养基:M0203)
生长特性:悬浮
细胞形态:淋巴母细胞
产品规格:1 x 10^6 cells/vial/T25
冻存条件:细胞保存液(货号:G0200)
保存条件:液氮存储
用户须知:
1、关于货期:除复苏细胞外,承诺货期1周内到(节假日顺延),大多数产品1-3天内发货。细胞产品因需要复苏后进行1-2代传代调整后等待细胞长满,期间进行微生物检测,货期1、2周左右。
2、关于订购:为了保证您的权益,下单前务必联系我司客户服务热线(021-55150076)。
细胞传代:
1.弃去培养液,用PBS洗涤1-2次;
2.加入1.0ml胰酶消化液,37℃消化,显微镜下观察细胞消化情况,若细胞回缩变圆、透亮、轻拍甁壁呈流沙样脱落,则迅速拿回操作台,加入完全培养液,终止消化并轻轻吹打细胞,使其变成单细胞悬液;
3.将细胞收集于离心管中离心1000rmp/5min,弃上清,轻弹管底,将细胞弹散;
4.加入新鲜培养液重悬细胞,进行传代、冻存;
5.如果没有特别说明,建议收到细胞后的第一次传代比例为1:2。
6.注意培养基PH值变化,定期换液(每周2-3次)。
安全性:
所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。
联系人:胡经理
电话:15921028707(微信同号)
:3407506161
:021-55150076
官网:https://whelab.com
公司邮箱:info@whelab.com
技术邮箱:tech@whelab.com
地址:上海宝山区呼兰西路129号8号楼4层
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
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An Epstein-Barr virus-negative Burkitt lymphoma cell line (sfRamos) secretes a prolactin-like protein during continuous growth in serum-free medium.
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Proc. Natl. Acad. Sci. U.S.A. 88:5413-5417(1991)
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DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
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Hemi- or homozygosity: a requirement for some but not other p53 mutant proteins to accumulate and exert a pathogenetic effect.
FASEB J. 7:951-956(1993)
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Variable IgH chain enhancer activity in Burkitt's lymphomas suggests an additional, direct mechanism of c-myc deregulation.
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High susceptibility of an Epstein-Barr virus-converted Burkitt's lymphoma cell line to cytotoxic drugs.
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p16/INK4a and p15/INK4b gene methylation and absence of p16/INK4a mRNA and protein expression in Burkitt's lymphoma.
Blood 91:1680-1687(1998)
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Bax is frequently compromised in Burkitt's lymphomas with irreversible resistance to Fas-induced apoptosis.
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The c-MYC allele that is translocated into the IgH locus undergoes constitutive hypermutation in a Burkitt's lymphoma line.
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Zander Balderud L., Bemark M.
Identification of genes deregulated during serum-free medium adaptation of a Burkitt's lymphoma cell line.
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Kang M.H., Smith M.A., Morton C.L., Keshelava N., Houghton P.J., Reynolds C.P.
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
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Comprehensive cytogenetic and molecular cytogenetic analysis of 44 Burkitt lymphoma cell lines: secondary chromosomal changes characterization, karyotypic evolution, and comparison with primary samples.
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Characterization of human cancer cell lines by reverse-phase protein arrays.
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