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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Drosophila, Dog, Hamster, Chicken, Monkey, Xenopus
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX11316
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, ELISA
- 靶点:
Monoclonal Anti-gamma-Tubulin recognizes an epitope located in the N-terminal amino acids 38-53 of gamma-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus gamma-tubulin.
- 抗体英文名:
gamma Tubulin antibody [GTU-88]
- 抗体名:
gamma Tubulin 抗体 [GTU-88]
- 规格:
100 μl
ICC/IF analysis of HeLa cells using GTX11316 gamma Tubulin antibody [GTU-88] at 1:5,000. Cells were fixed and permeabilized with methanol followed by acetone.
WB analysis of chicken fibroblasts using GTX11316 gamma Tubulin antibody [GTU-88].
Lane A : Antibody dilution 1:20,000
Lane B : Negative control (secondary antibody only)
WB analysis of (1) HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cell lysate using GTX11316 gamma Tubulin antibody [GTU-88] at 1:10,000.
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文献和实验Dewey EB et al., Mol Biol Cell 2017 (PMID:28747439)
Morii M et al., Sci Rep 2021 (PMID:33510346)
Wang EJ et al., Cells 2020 (PMID:32178256)
Chen WS et al., Sci Rep 2017 (PMID:28205572)
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
and sensitive to changes in both the chemical and mechanical environment. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins
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