ELISA试剂盒缓冲液 ELISA Buffer Set-

Application

Coating buffer: Use coating buffer to dilute the sample you will coat, add 100μL/well to high-bind plate.
Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate.
Blocking buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 4 times dilution.
Substrate solution: Add 100μL/well to plate.
Stop solution: Add 50μL/well to plate.

Storage

Store this set at 2-8℃, and protect from light. If precipitation occurs, filter to clarify before use.