BS3

IASYS-binding cuvette and getting KD

baseline (the changes of the signal response were smaller than 3 arc seconds in 10 minutes). (2) Aspirate and sodium phosphate buffer.Add 200 µl of BS3 solution (1mM,0.57 mg/ml)and wait for 15 minutes. (3) Aspirate the BS3 and add 200 µl sodium phosphate

Protein Interaction Analysis Using an IASYS Biosensor

buffer (10 mM,pH 7.7)to the cuvette and obtain a stable baseline (the changes of the signal response were smaller than 3 arc seconds in 10 minutes). Aspirate and sodium phosphate buffer.Add 200 µl of BS3 solution (1mM,0.57 mg/ml)and wait for 15 minutes

内源性FSTL1 通过激活钠‐钾泵引起膜超极化调节痛觉信息传递

的重组FSTL1 蛋白孵育培养的大鼠DRG 神经元，BS3 交联与细胞膜上受体结合的FSTL1，裂解细胞。细胞裂解物做Western blotting，用抗Myc 的抗体进行免疫共沉淀（IP），在140 kD 处发现阳性条带。于FSTL1 约40 kD，因此推断FSTL1 结合了约100 kD 的蛋白。相同的方法富集交联复合物，用质谱分析发现结合蛋白可能为NKA 的α1 亚基。放免实验和co‐IP 实验证明了与FSTL1 结合的蛋白正是NKA 的α1 亚基。 由于FSTL1 能通过清亮小泡