(3-Benzyloxycarbonyl-5-Nitroph

ELISA protocol

as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance

ELISA（酶联免疫吸附试验）

for HRP conjugates. Normally a 1:1000 dilution is sufficient. 7. Wash four times as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow

硅胶基硝化苯硼酸亲和色谱材料的合成及应用

of Chemical Physics,Chinese Academy of Sciences, Dalian 116023） 　　Abstract  A boronic acidsubstituted silica was synthesized by using a γglycidoxypropyltrimethoxysilane as the spacer arm and using a 3amino4nitrobenzeneboronic acid as the ligand. The 3amino