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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
见标签
- 库存:
现货
- 供应商:
鹿森生物
- CAS号:
57-00-1
- 规格:
50g
应用
生化/生理作用

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文献和实验is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
(Sigma A-9647) sddH2 O to 5 ml (aliquot and store at -20℃) 100 mM calcium chloride (CaCl2) : 1.48 g CaCl2-2H2 O ddH2 O to 100 ml autoclave to sterilize (store at 4deg.C).50 mM calcium chloride : 0.74 g CaCl2-2H2 O ddH2 O to 100 ml autoclave
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