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- H0915-5MG
- 2025年09月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
见标签
- 库存：
现货
- 供应商：
鹿森生物
- CAS号：
747-36-4
- 规格：
5mg
应用
- 用作自噬的抑制剂，以处理细胞进行凋亡测定[1]用于研究吡柔比星在膀胱癌细胞系中诱导自噬的作用[2]
- 用于通过免疫荧光测定来评估羟氯喹对登革病毒-2 （DENV-2）感染的抗病毒机制[3]
- 用于抑制溶酶体与自噬体的融合[4]
生化/生理作用
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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
expression vectors with strong promoter such as immediate early CMV (pcDNA3.1 etc.) or human EF-1a (pEF1/V5-His etc.). pBJ-5/GS vector also gives you high expression but requires additional cloning strategy. Materials Medium Sigma provides
real time sybergreen concentration-Real-Time PCR
, but to buy it in a kit - like Sigma SybrGReen jumstart ready mix which is what i use (lots of companies have these kits eg. sigma, invitrogen). only because it reduces the error you may have in adding it seperately. and with real time, it is so sensitive
Establishment of Stable Transfectant of CHO Lec Cells
) by yourself but this cannot be used as serum-free medium. Recipe for 10 lt of J/J medium: To 9 lt of mili-Q water, add MEM w/o Gln (GIBCO, #11700-077). Pwdr for 10 lt. NaHCO3 , 27.75g glucose: 30 g nucleosides: adenosine (Sigma A-4036), 70mg
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