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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
见标签
- 库存:
现货
- 供应商:
鹿森生物
- 规格:
100ul
一般描述

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文献和实验Staining for Surface Receptors
coverslips. Equilibrate in incubator (~ 25'). 4. Add primary antibody at 1:500 to 1:1000 dilution to media for 30'. Example antibodies: M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; 12CA5 mouse anti-HA (Roche )1:1000
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
according to my protocol. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP
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