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Sigma:Anti-HumanIgG(γ-chainspe

cific)antibody,Mousemonoclonal,SAB4200713-100ul,
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  • ¥99 - 999
  • Sigma
  • 进口
  • SAB4200713-100ul
  • 2025年09月13日
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    • 文献和实验
    • 技术资料
    • 保存条件

      常温

    • 保质期

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    • 库存

      现货

    • 供应商

      鹿森生物

    • 规格

      100ul

    一般描述

    Anti-Human IgG (γ-chain specific) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG. Immunoglobulins (Igs) are produced by B lymphocytes and secreted into the plasma. Immunoglobulins can be divided into five main classes/isotypes which are IgA, IgD, IgE, IgG, and IgM. IgG class identity is determined by class-specific sequences in the Fc region of the heavy chain. IgG antibody class are the most abundant immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4). The IgG subclasses differ in their physical and chemical properties. Their distribution pattern is found to be age dependent and every subclass has a specific biological function.

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    图标文献和实验
    相关实验
    • Staining for Surface Receptors

      coverslips. Equilibrate in incubator (~ 25'). 4. Add primary antibody at 1:500 to 1:1000 dilution to media for 30'. Example antibodies: M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; 12CA5 mouse anti-HA (Roche )1:1000

    • Actin Capture Assay

      . For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA

    • Actin Capture Assay

      according to my protocol. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP

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