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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
见标签
- 库存:
现货
- 供应商:
联硕
- CAS号:
108-59-8
- 规格:
1g
一般描述
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文献和实验Solutions for Tissue/Cell Digestion
into 250 ml media bottles, 100 ml (10 bottles) each. 4. Store at -20 for daily use and -70℃ for long term. Trypsin 0.25% Materials Trypsin 1:300 (GIBCO 840-7073) 2.5 g TD 1000 ml
Solutions for Tissue/Cell Digestion
O) or 7 ml of 100 mM Tris HCl 3.2 g Tris base 0.59 g pH should be ~7.5. Store in refrigerator. STE 4000 ml 1000 ml NaCl 150 mM 35.1 g 8.8 g Tris HCl 44 mM
Alternative Cell Culture Systems
and incubated under the culture conditions appropriate for the cell line. Cultures should be stirred at 100-250 revolutions per minute. Spinner flask systems designed to handle culture volumes of 1-12 liters are available from Techne, Sigma, and Bellco, e.g
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