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一般描述
胰蛋白酶是一种丝氨酸蛋白酶,可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联 苯氯甲基 酮 (TPCK) 处理,可使外源性糜蛋白酶失活。每个包装含8个小瓶,每瓶含25 μg。
应用
TPCK处理的胰蛋白酶已被用于:
作为杜尔贝科改良伊格尔培养基(DMEM)的补充剂,用于上皮样猪肾细胞系(LLC-PK1) 的猪Delta冠状病毒(PDCoV)感染实验[1]
分离人脐静脉内皮细胞(HUVEC) 用于膜联蛋白-V/碘化丙啶 (PI) 染色测定[2]
用于多循环复制动力学的最小必需培养基 (MEM)[3]
生化/生理作用
N-对-对甲苯磺酰基-L-苯丙氨酸氯甲基 酮(TPCK) 可用作糜蛋白酶的不可逆抑制剂。胰蛋白酶诱导人纤维细胞分化。[4]胰蛋白酶被广泛用于蛋白质组学中的蛋白质样品消化。在细胞培养中,进行胰蛋白酶消化是为了使贴壁细胞彼此分开并离开培养容器壁。
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文献和实验Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas.
Devraj Basu et al.
Cell cycle (Georgetown, Tex.), 10(12), 2008-2016 (2011-05-12)
Multiplex PCR: Critical Parameters and Step
), or quantitative assays (10) and reversetranscription PCR (7). The role of various reagents in PCR has been discussed (3,9,12,13), and protocols for multiplex PCR have been described by a number of groups. However, few studies (5,15) have presented
Multiplex PCR: Critical Parameters and Step-by-Step Protocol
), or quantitative assays (10) and reversetranscription PCR (7). The role of various reagents in PCR has been discussed (3,9,12,13), and protocols for multiplex PCR have been described by a number of groups. However, few studies (5,15) have presented
Multiplex Illumina Sequencing Using DNA Barcoding
. This unit contains a barcoding protocol for the preparation of up to 96 ChIP samples for multiplex sequencing in a single flow cell lane on the Illumina platform. This strategy may be extended to even larger numbers of samples and may also be generalized
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