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应用
本试剂盒适用于常规细胞膜标记。PKH67具有长于PKH1和PKH2的脂肪炭尾,后两者是另外两种之前常用于体外和体内细胞示踪的绿色染料。由于炭尾长度更长,内部研究已经证明PKH67比PKH2由更少的细胞间转移。
在采用PKH1和PKH2进行的体内研究中,荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征,因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示,PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输,结果表明PKH67还可用于中等时长的体内跟踪研究。
用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒已用于:
标记并进一步研究在三阴性乳腺癌状况下从细胞中排出的外泌体。[1]
标记凋亡性细胞,[2]用于研究ανβ5受体在凋亡性细胞的结合和内化中的作用。[3]
在荧光成像中。
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文献和实验alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Applications for Green Fluorescent Protein in Cell Signaling
The discovery of green fluorescent protein (GFP) as a naturally fluorescing protein and its subsequent modification to allow easy detection with standard fluorescent equipment such as the fluorescence-activated cell sorter (FACS
Cell Lineage Analysis: Applications of Green Fluorescent Protein
The ideal cell lineage marker is one that can be visualized in living tissues without perturbing development. Exogenously applied dyes are very useful, but in many instances there would be advantages to an endogenously expressed marker. Green
Live Imaging with Green Fluorescent Protein
If developmental biologists were given the chance to design the perfect cell-or tissue-specific marker, they would ensure that it had several properties. First, it would function in living animals, eliminating the need for fixation
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