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TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which activates the cell′s apoptotic machinery. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.
外形
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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文献和实验Macrophage-expressed IFN-β contributes to apoptotic alveolar epithelial cell injury in severe influenza virus pneumonia.
Katrin Högner et al.
PLoS pathogens, 9(2), e1003188-e1003188 (2013-03-08)
跟踪信息素 trail pheromone，trail markingpheromone
蚁、蜜蜂、白蚁等社会性昆虫，从巢出来寻找猎物，归巢时，在路上涂擦分泌物作为道路标志，其他个体跟踪前往便可达到目的地。这种分泌物称为跟踪信息素。蚁是从杜氏腺（ Dufour′ s gland）和毒腺分泌出来的，蜜蜂是从发香腺分泌出来的，其组成成分经分离鉴定为牛儿醇（ geraniol）等。白蚁则从腹板腺（ sternal gland）分泌的。跟踪信息素以外的物质，也有具有跟踪作用的化合物。
(6 -10 ). Thus, treatment with TRAIL might be a promising therapeutic approach for many solid tumors.
Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy
activity of TRAIL such that larger, more established tumors could be eradicated as efficiently as smaller tumors. The information presented here describes the production of an adenoviral vector engineered to carry the complementary DNA (cDNA) for murine
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