- ￥99 - 999
- Sigma
- 进口
- 83580-1G
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
见标签
- 库存：
现货
- 供应商：
联硕
- CAS号：
50-55-5
- 规格：
1g
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生化/生理作用
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文献和实验CHAPS Insoluble Floating Fraction Preparation
7.5, 50 mM NaCl, 2 mM EDTA). They were finally resuspended in TEBP (TEB plus various protease inhibitors: 1x PIC I and 1x PIC II) to 2 x 108 /ml. CHAPS powder was added at 20 mg/ml. After gentle mixing, the lysed cells were kept on ice for 3-5
A quick RNA mini-prep for Neurospora mycelial cultures
temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM EDTA, pH 8.0, 2 SDS; autoclave and add 1 mM ATA and 14 mM ß-mercaptoethanol. ATA=aurintricarboxylic acid, ammonium salt (Sigma #A0885, St. Louis, MO)] 5. Thaw the powder in E buffer in 42°C
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
). More can be cut if additional studies (e.g. immunohistochemistry) are being considered. Methyl Green (see protocol below). If the histopathology is at all difficult, photos are taken of areas to be dissected on both the methyl green and H&E slides
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