CrystalCap HT™, 0.025 - 0.05 m

蛋白偶联到磁性MagPlex™微球的方法

  Sample Protopcol  for  Two-Step Carbodiimide  Coupling   of  Protein to MagPlex™ Magnetic  Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend

蛋白样品制备中各类杂质和杂蛋白的去除

/冲洗缓冲液 (10 mM Imidazole, 0.3M NaCl, 0.05M sodium phosphate (pH8.0)) 100 ml His 洗脱缓冲液 (0.25M Imidazole, 0.3M NaCl, 0.05M sodium phosphate (pH8.0)) 产品特点： ● 用于纯化 6XHis 标签蛋白 ● 高载量，大于 50 mg/ml ● 配基密度，每毫升树脂结合 20-40μmoles 镍离子 ●

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™