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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Histone H3K4 Monomethyl Polyclonal Antibody (50 ug)
- 供应商:
上海西宝
- 规格:
50 ug
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文献和实验," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
, immunocytochemistry) does not automatically mean the antibody is suitable for ChIP; that has to be tested. Antibodies can be available as polyclonal or monoclonal preparations. Monoclonal antibodies have a high specificity compared to polyclonal sera
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